This is a eDNA report of all the analysis performed for the SubBioMon program. All the sampling were performed using Sylphium filter and by amplifying both the CO1 and the 28S marker. The amplification were then sequenced using Nanopore sequencing and clusterized using the Natrix2 pipeline using an identity treshold of 97% and minimum Q score of 7. The OTUs were then blast over the NCBI database

Albania

Sardinia

Locoli

2024

September
NMDS

CO1 metabarcoding

Krona

Note that this Krona plot has been made withe the best blast identity possible. Some of the OTUs did not have good blast using NCBI database. For species presence absence refers to the “species presence” tab

Detected Species

Detected species has been calculated by removing every OTU that had less than 90 query coverage and less than 99% pident. The pident treshold has been chosen following Egeter et al. 2022. The coverage treshold has been chosen arbitrarly


Comment

A lot of site are contaminated by Homo sapiens DNA coming from the speleodivers that retrieved the water

Minopterus has been seen and detected at the entry of the cave, as well as Niphargus

Stennaselus has also been caught using traps and has been detected in the upstream part of the aquifer

Aqueduct monthly monitoring

2024

June
July
August
September
October

Luxembourg