This is a eDNA report of all the analysis performed for the SubBioMon program. All the sampling were performed using Sylphium filter and by amplifying both the CO1 and the 28S marker. The amplification were then sequenced using Nanopore sequencing and clusterized using the Natrix2 pipeline using an identity treshold of 97% and minimum Q score of 7. The OTUs were then blast over the NCBI database
CO1 metabarcoding
Note that this Krona plot has been made withe the best blast identity possible. Some of the OTUs did not have good blast using NCBI database. For species presence absence refers to the “species presence” tab
Detected species has been calculated by removing every OTU that had less than 90 query coverage and less than 99% pident. The pident treshold has been chosen following Egeter et al. 2022. The coverage treshold has been chosen arbitrarly
Comment
A lot of site are contaminated by Homo sapiens DNA coming from the speleodivers that retrieved the water
Minopterus has been seen and detected at the entry of the cave, as well as Niphargus
Stennaselus has also been caught using traps and has been detected in the upstream part of the aquifer